Effects Of Ph On The Enzyme Activity

Aim: To investigate the effects of changing in PH on the activity of enzyme (trypsin). Hypothesis: Alternative hypothesis: As the PH level goes up, the enzyme activity of trypsin will speed up. The pursual graph is the prediction supporting my hypothesis. Introduction: Biology background (with theories) What be enzymes? Enzymes act as biological catalysts; they make chemical reactions to bet quicker and remains unchanged when the reaction finishes. accordingly enzymes be used to speed up biological reactions in body. They great deal be working within cells and outside cells catalyzing metabolous reactions in human bodies. Enzymes are globular proteins; therefore they are meltable and easily transported in blood. Enzyme has an active site in a specific see because of its tertiary structure. Enzyme works for book adhere with substance of a specific organize that fits in it and hurly beefy down the substance. Enzymes denature due to vari ous factors. The temperature, PH level, and the parsimony of the substance influence enzyme activities. When these factors vary, enzymes may change in shape so it will not be able to bind to the specific substance anymore. What is trypsin? Trypsin is produced in the pancreas as typsinogen originally allowing metabolous discover.

It is used widely in various biotechnological processes because it is very well-heeled to be purified. Method: Material: Trypsin Casein Water bathe tub (to keep the temperature perpetual) Colorimeter (to prize the rate of enzyme activity) Thermometer demo nstrate Tubes (to contain the casein and th! e buffer solution) Stop watch (to control and measure the time) Distilled water (to mix with trypsin to produce the buffer solution) attempt supply rack (to prevent the tubes rolling and smashing) Protocol: allot 2.5 cm³ of 5% with reconstituted casein in 6 of the test tubes (Tube A) derriere the test tubes into the water bath at a constant temperature for 5 minutes....If you want to get a full(a) essay, influence it on our website: OrderCustomPaper.com

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